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[摘要]：In this study, we explored the use of fosfomycin modified microparticulate zirconia (FZ) as a novel hydrophobic interaction chromatography (HIC) stationary phase for proteins separation. The results showed that on the FZ stationary phase, the basic proteins such as lysozyme, ribonuclease-A, and a-chymotrypsin, presented a retention mechanism that well agreed with the theory of hydrophobic interaction. The undesirable cation exchange and ligand exchange interactions that usually resulted from the complex surface chemistry of zirconia matrix, could be effectively suppressed due to the phosphonate modification and moderately high salt concentration used in the mobile phases. A few basic proteins achieved good separations by the gradient elution mode with decreasing salt concentration, and high mass recoveries, as well as loading capacity for these proteins obtained on the FZ column. This demonstrates that the FZ stationary phase would be a complement for biocompatible HIC materials, especially for basic proteins, and allows zirconia based supports are more useful for bio-separation. |
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