[摘要]:This paper presents an improved direct binary search (DBS)-based algorithm for generating holograms to holographic optical tweezers. The simulations show that the improved algorithm greatly enhances computation speed while maintaining high hologram efficiency and high-intensity homogeneous target spots. The improved algorithm was applied to generate holographic optical tweezers in several experiments. The experiments demonstrate that real-time trap and manipulation can be realized with the improved algorithm if the number of trapped microparticles is small. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.OE.51.1.015801]