[摘要]：D-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media D-lactate concentrations greater than 5-10 mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous D-lactate dehydrogenase (D-LDH) detoxify D-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, D-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on D-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
