【文章名】Insights into subtle conformational differences in the substrate-binding loop of fungal 17 beta-hydroxysteroid dehydrogenase: a combined structural and kinetic approach
Insights into subtle conformational differences in the substrate-binding loop of fungal 17 beta-hydroxysteroid dehydrogenase: a combined structural and kinetic approach
[摘要]：The 17 beta-HSD (17 beta-hydroxysteroid dehydrogenase) from the filamentous fungus Cochliobolus lunatics (17 beta-HSDcl) is a NADP(H)-dependent enzyme that preferentially catalyses the interconversion of inactive 17-oxo-steroids and their active 17 beta-hydroxy counterparts. 17 beta-HSDcl belongs to the SDR (short-chain dehydrogenase/reductase) superfamily. It is currently the only fungal 17 beta-HSD member that has been described and represents one of the model enzymes of the cP1 classical subfamily of NADPH-dependent SDR enzymes. A thorough crystallographic analysis has been performed to better understand the structural aspects of this subfamily and provide insights into the evolution of the HSD enzymes. The crystal structures of the 17 beta-HSDcl apo, holo and coumestrol-inhibited ternary complex, and the active-site Y167F mutant reveal subtle conformational differences in the substrate-binding loop that probably modulate the catalytic activity of 17 beta-HSDcl. Coumestrol, a plant-derived non-steroidal compound with oestrogenic activity, inhibits 17 beta-HSDcl [IC(50) 2.8 mu M; at 100 mu M substrate (4-oestrene3,17-dione)] by occupying the putative steroid-binding site. In addition to an extensive hydrogen-bonding network, coumestrol binding is stabilized further by pi-pi stacking interactions with Tyr(212). A stopped-flow kinetic experiment clearly showed the coenzyme dissociation as the slowest step of the reaction and, in addition to the low steroid solubility, it prevents the accumulation of enzyme-coenzyme-steroid ternary complexes.
