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[摘要]：gal-1 (galectin-1) mediates cell-cell and cell-extracellular matrix adhesion, essentially by interacting with beta-galactoside-containing glycans of cell-surface glycoconjugates. Although most structural studies with gal-1 have investigated its binding to simple carbohydrates, in particular lactose and N-acetyl-lactosamine, this view is limited, because gal-1 functions tit the cell surface by interacting with more complex glycans that are heterogeneous in size and composition. In the present Study we used NMR spectroscopy to investigate the interaction of human gal-1 with a large (120 kDa) complex glycan, GRG (galactorhamnogalacturonate glycan), that contains non-randomly distributed mostly terminal beta(1 -> 4)-linked galactose side chains. We used N-15-H-1-HSQC (heteronuclear single quantum coherence) NMR experiments with N-15-enriched gal-1 to identify the GRG-binding region oil gal-1 and found that this region covers a large surface area on gal-1 that includes the quintessential lactose-binding site and runs from that site through a broad valley or cleft towards the dimer interface. HSQC and pulsed-field-gradient NMR diffusion experiments also show that gal-1 binds GRG with a gal-1:GRG stoichiometry of about 5:1 (or 6:1)and with average macroscopic and microscopic equilibrium dissociation constants (K-d) of 8 x 10(-6) M and 40 x 10(-6) M (or 48 x 10(-6) M) respectively, indicating stronger binding than to lactose (K-d = 520 x 10(-6) M). Although gal-1 may bind GRG in various ways, the glycan call be competed for by lactose, suggesting that there is one major mode of interaction. Furthermore, even though terminal motifs on GRG tire Gal-beta(1 -> 4)-Gal rather than the traditional Gal-beta(1 -> 4)Glc/GlcNAc (where GlcNAc is N-acetylglucosamine), we show that the disaccharide Gal-beta(1 -> 4)-Gal can bind gal-1 at the lactose-binding domain. In addition, gal-1 binding to GRG disrupts inter-glycan interactions and decreases glycan-mediated solution viscosity, a glycan decongestion effect that may help explain why gal-1 promotes membrane fluidity and lateral diffusion of glycoconjugates within cell membranes. Over-all, our results provide an insight into the function of galectin in situ and have potential significant biological consequences. |
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