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Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens

  作者 Carette, JE; Guimaraes, CP; Varadarajan, M; Park, AS; Wuethrich, I; Godarova, A; Kotecki, M; Cochran, BH; Spooner, E; Ploegh, HL; Brummelkamp, TR  
  选自 期刊  Science;  卷期  2009年326-5957;  页码  1231-1235  
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[摘要]Loss-of-function genetic screens in model organisms have elucidated numerous biological processes, but the diploid genome of mammalian cells has precluded large-scale gene disruption. We used insertional mutagenesis to develop a screening method to genera

 
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