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[摘要]：RACK1 (receptor for activated C kinase 1) is an abundant scaf-folding protein, which binds active PKC beta II (protein kinase C beta II) increasing its activity in vitro. RACK1 has also been described as a component of the small ribosornal subunit, in proximity to the mRNA exit channel. In the present Study we tested the hypothesis that PKC beta II plays a specific role in translational control and verified whether it may associate with the ribosomal machinery. We find that specific inhibition of PKC beta I/II reduces translation as well as global PKC inhibition, but without affecting phosphorylation of mTOR (mammalian target of rapamycin) targets. These results suggest that PKC beta II acts as a specific PKC isoform affecting translation in an mTOR-independent fashion, possibly close to the ribosomal machinery. Using far-Western analysis, we found that PKC beta II binds ribosomes in vitro. Coimmunoprecipitation studies indicate that a small but reproducible pool of PKC beta II is associated with membranes containing ribosomes, Suggesting that in vivo PKC 11 may also physically interact with the ribosornal machinery. Polysomal profiles show that stimulation of PKC results in an increased polysomes/80S ratio, associated with a shift of PKC beta II to the heavier part of the gradient. A RACK1-derived peptide that inhibits the binding of active PKC beta II to RACK1 reduces the polysomes/80S ratio and methionine incorporation, suggesting that binding of PKC beta II to RACK1 is important for PKC-mediated translational control. Finally, down-regulation of RACK I by siRNA (small interfering RNA) impairs the PKC-mediated increase of translation. Taken together the results of the present study show that PKC beta II can act as a specific PKC isoform regulating translation, in an mTO-independent fashion, possibly close to the ribosomal machinery. |
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