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[摘要]：Two genes, prsI and arsI, are located divergently next to the sigI gene encoding alternative sigma factor sigma(I) of Streptomyces coelicolor A3(2). The similarity of prsI and ArsI to anti-sigma and anti-anti-sigma factors, respectively, suggests that both putative regulators may be involved in regulation of sigma(I). By using a combination of several approaches including bacterial two-hybrid assays, pull-down assay and visualization of the complex by native polyacrylamide electrophoresis, we demonstrated that prsI specifically interacts with sigma(I) and ArsI. In vitro phosphorylation demonstrated that prsI serves as a specific kinase for its putative partner, ArsI, and negatively regulates its activity. The sigI gene was deleted in the S. coelicolor M145 strain without obvious effect on growth, stress response and differentiation. Complex transcriptional analyses of sigI, prsI, and am! revealed that sigI is directed by a single promoter induced by osmotic stress, arsI is directed by a single constitutive promoter, and prsI is directed by two tandem promoters, one constitutive and the second one induced by osmotic stress. None of the determined promoters was dependent upon sigma(I), sigma(B) and sigma(H). These data suggested a role of sigma(I) in the osmotic stress response and its regulation by a partner switching mechanism through the anti-sigma factor prsI and its antagonist, anti-anti-sigma factor ArsI. (C) 2011 Elsevier B.V. All rights reserved. |
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