Surface-Initiated Polymerization of 2-Hydroxyethyl Methacrylate from Heterotelechelic Oligoperoxide-Coated gamma-Fe(2)O(3) Nanoparticles and their Engulfment by Mammalian Cells

[摘要]：Novel heterotelechelic poly(N-vinylpyrrolidone) (CPA-PVP-IBMB) oligoperoxide containing anchoring carboxyl group from 4-cyanopentanoic acid (CPA) and peroxide end-group from 1-isopropyl-3(4)-[1-(tert-butyl peroxy)-1-methylethyl]benzene (IBMB) chain transfer agent was synthesized and investigated as a surface-active macroinitiator for tailored functionalization of maghemite (gamma-Fe(2)O(3)) nanoparticles. CPA-PVP-IBMB initiated polymerization of 2-hydroxyethyl methacrylate (HEMA) in ethanol yielding CPA-PVP-block-poly(2-hydroxyethyl methacrylate) (CPA-PVP-block-PHEMA) copolymer. gamma-Fe(2)O(3) nanoparticles obtained by precipitation technique were modified by the CPA-PVP-IBMB macroinitiator and CPA-PVP-block-PHEMA copolymer by "grafting-to" approach to the surface using carboxyl end groups of both polymers. Second method, "grafting-from" approach, consisted in polymerization of HEMA initiated from the surface of CPA-PVP-IBMB-coated gamma-Fe(2)O(3) nanoparticles yielding CPA-PVP-IBMB-coated gamma-Fe(2)O(3)/PHEMA nanoparticles. Tests with mice leukemia L1210 cells confirmed nontoxicity of CPA-PVP-IBMB-coated gamma-Fe(2)O(3)/PHEMA nanoparticles. CPA-PVP-IBMB-coated gamma-Fe(2)O(3)/PHEMA nanoparticles were easily engulfed by murine macrophages J774.2 and magnetically separated from cells without phagocytic activity.

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