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[摘要]:The authors have synthesized Singapore Green 1 and 2 (I, R = Me and (CH2)5OH resp.), structural hybrids of Tokyo Green and Rhodamine 110. The new dyes are green light-emitting substitutes for 7-aminocoumarin, a blue fluorescent dye widely used in enzymic assays. Singapore Green-conjugated peptide substrates were successfully synthesized and used in microarray-based protease substrate profiling and live-cell imaging expts. |
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