[摘要]：Haruan (Channa striatus) is in great demand in the Malaysian domestic fish market. In the present study, mtDNA cyt b was used to investigate genetic variation of C striatus among populations in Peninsular Malaysia. The overall population of C striatus demonstrated a high level of haplotype diversity (h) and a low-to-moderate level of nucleotide diversity (pi). Analysis of molecular variance (AMOVA) results showed a significantly different genetic differentiation among 6 populations (F-sT = 0.37566, P = 0.01). Gene flow (Nm) was high and ranged from 0.32469 to infinity (infinity). No significant relationship between genetic distance and geographic distance was detected. A UPGMA tree based on the distance matrix of net interpopulation nucleotide divergence (d(A)) and haplotype network of mtDNA cyt b revealed that C. striatus is divided into 2 major clades. The neutrality and mismatch distribution tests for all populations suggested that C striatus in the study areas had undergone population expansion. The estimated time of population expansion in the mtDNA cyt b of C. striatus populations occurred 0.72-6.19 million years ago. Genetic diversity of mtDNA cyt b and population structure among Haruan populations in Peninsular Malaysia will be useful in fisheries management for standardization for Good Agriculture Practices (GAP) in fish-farming technology, as well as providing the basis for Good Manufacturing Practices (GMP). (C) 2011 Elsevier B.V. All rights reserved.
