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[摘要]：The aim of the present study was to develop a new biopolymer to increase endothelial progenitor cells (EPC) survival and amplification. As a cell culture platform, bone marrow-derived cells (BMDC) were used to investigate the biocompatibility of chitosan-phosphorylcholine (CH-PC). On CH-PC, BMDC were found in colonies with a mortality rate similar to that of fibronectin (FN), the control matrix. Adhesion/proliferation assays demonstrated a greater number of BMDC on CH-PC after 7 days with an amplification phase occurring during the second week. Difference in adhesion mechanisms between (CH-PC) and the control FN matrix suggest distinctive cell retention ability. Confocal microscopy analyses confirmed that (CH-PC) supported the survival/differentiation of endothelial cells. Moreover, flow cytometry analyses demonstrated that, (CH-PC) increased the percentage of progenitor cells (CD117(+)CD34(+)) (7.1 +/- 0.8%, FN: 4.1 +/- 0.8%) and EPC (CD117(+)CD34(+)VEGFR-2(+)CD31(+)) (233 +/- 0.6%, FN: 0.25 +/- 0.1%), while the mesenchymal stem cell fraction (CD44(+)CD106(+)CD90(+)) was decreased (0.07 +/- 0.01%, FN: 0.55 +/- 0.22%). Polymeric substrate CH PC might provide a suitable surface to promote the amplification of EPC for future vascular therapeutic applications. (C) 2011 Elsevier Ltd. All rights reserved. |
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