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[摘要]：Noxa1 was discovered as an activating factor for Nox1, an O-2(-)-generating enzyme. Subsequent studies have shown that Noxa1 is colocalized with Nox2 in several cell types, including vascular cells. Nox2 activation by Noxa1 has been examined in reconstituted model cells. However, little is known about the kinetic properties of Noxa1 in Nox2 activation. In the present study, we used purified cyt.b(558)(Nox2 plus p22(phox)), Rac(Q61L), and Noxol to examine the ability of Noxal to activate Nox2. In the pure reconstitution system, Noxa1 activated Nox2 with lower efficiency than p67(phox), a canonical activator of Nox2. The EC50 value of Noxa1 was considerably higher than that of p67(phox). The V-max value with Noxal and Noxol was one-third of that with p67(phox) and p67(phox). The EC50 value of Noxol or Rac(Q61L) was also higher when Noxal was used. The affinity of FAD for the oxidase and the stability of the active complex were remarkably low when Noxal and Noxo1 were used compared with p67(phox) and p67(phox). The stability was not improved by fusion of Noxa1 with Rac(Q61L). These findings show that Noxa1 has quite different kinetic properties from p67(phox) and suggest that Noxa1 may function as a moderate activator of Nox2. (C) 2012 Elsevier Inc. All rights reserved. |
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