| |
作者 |
Yang, HN; Park, JS; Woo, DG; Jeon, SY; Do, HJ; Lim, HY; Kim, JH; Park, KH |
|
| |
[摘要]：In this study, to drive efficient adipogenic differentiation, the adipogenic transcription factors C/EBP-alpha and C/EBP-beta fused to green fluorescent protein (GFP) or red fluorescent protein (RFP) were complexed with poly-ethyleneimine (PEI) coupled with biodegradable PLGA nanospheres and delivered to human mesenchymal stem cell (hMSC). FACS analysis revealed that the transfection efficiency of C/EBP-alpha, C/EBP-beta, or both genes complexed with PEI-coated PLGA nanospheres was 12.59%, 21.74%, and 28.96% of hMSCs. Expression and localization of C/EBP-alpha and C/EBP-beta were confirmed by Western blotting and confocal laser microscopy. Overexpression of exogenous C/EBP-alpha and C/EBP-beta significantly elevated adipogenic differentiation processes as indicated by RT-PCR, real-time PCR, Western blotting, histology, and immunofluorescence microscopy. During adipogenesis, PEI-coupled PLGA nanospheres complexed with C/EBP-alpha and C/EBP-beta greatly increased the adipogenic capability of in vitro cultured cells, as well of in vivo transplanted cells. The expression of genes and proteins specific to adipogenic differentiation in hMSCs was significantly elevated compared to the controls. (C) 2011 Elsevier Ltd. All rights reserved. |
|
