[摘要]:High-throughput manipulation of microparticles can be efficiently accomplished using electrokinetic effects. In this contribution, we demonstrate the two-dimensional investigation of internal space-charge fields inside a bismuth silicon oxide (BSO) crystal and their use for optically mediated particle trapping. The magnitude of the internal fields as well as the time constant for its build-up are measured by Zernike phase contrast and digital holography. The fast response time of a BSO crystal at very low light powers enables real-time generation of high electric field gradients. We demonstrate that this photoconductive material facilitates both electrophoretic and dielectrophoretic trapping of particles on an accessible surface. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4704361]