- A Method for Helical RNA Global Structure Determination in Solution Using Small-Angle X-Ray Scattering and NMR Measurements
[作者:Wang, JB; Zuo, XB; Yu, P; Xu, H; Starich, MR; Tiede, DM; Shapiro, BA; Schwieters, CD; Wang, YX,期刊:Journal of Molecular Biology, 页码:717-734 , 文章类型: Article,,卷期:2009年393-3]
- We report a "top-down" method that uses mainly duplexes' global orientations and overall molecular dimension and shape restraints, which were extracted from experimental NMR and small-angle X-ray scattering data, respect...
- Metal Ion Dependence of Cooperative Collapse Transitions in RNA
[作者:Moghaddam, S; Caliskan, G; Chauhan, S; Hyeon, C; Briber, RM; Thirumalai, D; Woodson, SA,期刊:Journal of Molecular Biology, 页码:753-764 , 文章类型: Article,,卷期:2009年393-3]
- Positively charged counterions drive RNA molecules into compact configurations that lead to their biologically active structures. To understand how the valence and size of the cations influences the collapse transition i...
- Cholesterol Regulates Assembly of Human Islet Amyloid Polypeptide on Model Membranes
[作者:Cho, WJ; Trikha, S; Jeremic, AM,期刊:Journal of Molecular Biology, 页码:765-775 , 文章类型: Article,,卷期:2009年393-3]
- Amylin, a 37-aa pancreatic hormone, is the major constituent of islet amyloid, a hallmark of type II diabetes mellitus. Recent studies have revealed a pivotal role of anionic phospholipids in membrane-catalyzed amylin fi...
- Crystal Structures of Different Substates of Bacteriorhodopsin's M Intermediate at Various pH Levels
[作者:Yamamoto, M; Hayakawa, N; Murakami, M; Kouyama, T,期刊:Journal of Molecular Biology, 页码:559-573 , 文章类型: Article,,卷期:2009年393-3]
The hexagonal P622 crystal of bacteriorhodopsin, which is made up of stacked membranes, is stable provided that the precipitant concentration in the soaking solution is higher than a critical value (i.e., 1.5 M ammoni...
- DARPins as Bispecific Receptor Antagonists Analyzed for Immunoglobulin E Receptor Blockage
[作者:Eggel, A; Baumann, MJ; Amstutz, P; Stadler, BM; Vogel, M,期刊:Journal of Molecular Biology, 页码:598-607 , 文章类型: Article,,卷期:2009年393-3]
- The concept of multispecific antibodies is of high therapeutic interest but has failed to produce pharmaceutical products due to the poor biophysical properties of such molecules. Here, we propose an alternative and simp...
- Determinants for the Activation and Autoinhibition of the Diguanylate Cyclase Response Regulator WspR
[作者:De, N; Navarro, MVAS; Raghavan, RV; Sondermann, H,期刊:Journal of Molecular Biology, 页码:619-633 , 文章类型: Article,,卷期:2009年393-3]
- The bacterial second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) controls secretion, cell adhesion, and motility, leading to biofilm formation and increased cytotoxicity. Diguanylate cyclases ...
- Receiver Domains Control the Active-State Stoichiometry of Aquifex aeolicus sigma(54) Activator NtrC4, as Revealed by Electrospray Ionization Mass Spectrometry
[作者:Batchelor, JD; Sterling, HJ; Hong, E; Williams, ER; Wemmer, DE,期刊:Journal of Molecular Biology, 页码:634-643 , 文章类型: Article,,卷期:2009年393-3]
- A common challenge with studies of proteins in vitro is determining which constructs and conditions are most physiologically relevant. sigma(54) activators are proteins that undergo regulated assembly to form an active A...
- The Design and Characterization of Oligospecific Antibodies for Simultaneous Targeting of Multiple Disease Mediators
[作者:Dimasi, N; Gao, CS; Fleming, R; Woods, RM; Yao, XT; Shirinian, L; Kiener, PA; Wu, H,期刊:Journal of Molecular Biology, 页码:672-692 , 文章类型: Article,,卷期:2009年393-3]
- Monoclonal antibodies are traditionally used to block the function of a specific target in a given disease. However, some diseases are the consequence of multiple components or pathways and not the result of a single med...
- Structure of the Mature Streptococcal Cysteine Protease Exotoxin mSpeB in Its Active Dimeric Form
[作者:Olsen, JG; Dagil, R; Niclasen, LM; Sorensen, OE; Kragelund, BB,期刊:Journal of Molecular Biology, 页码:693-703 , 文章类型: Article,,卷期:2009年393-3]
- Invasive infections of Streptococcus pyogenes are dependent on the cysteine protease streptococcal pyrogenic exotoxin B. Previous structures of the enzyme have not disclosed the proper active-site configuration. Here, th...
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