- Ferric, not ferrous, heme activates RNA-binding protein DGCR8 for primary microRNA processing
[作者:Barr, I; Smith, AT; Chen, YQ; Senturia, R; Burstyn, JN; Guo, F,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1919-1924 , 文章类型: Article,,卷期:2012年109-6]
- The RNA-binding protein DiGeorge Critical Region 8 (DGCR8) and its partner nuclease Drosha are essential for processing of microRNA (miRNA) primary transcripts (pri-miRNAs) in animals. Previous work showed that DGCR8 for...
- Transforming growth factor beta-activated kinase 1 (TAK1)-dependent checkpoint in the survival of dendritic cells promotes immune homeostasis and function
[作者:Wang, YY; Huang, GH; Vogel, P; Neale, G; Reizis, B; Chi, HB,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:E343-E352 , 文章类型: Article,,卷期:2012年109-6]
- Homeostatic control of dendritic cell (DC) survival is crucial for adaptive immunity, but the molecular mechanism is not well defined. Moreover, how DCs influence immune homeostasis under steady state remains unclear. Co...
- Crystal structures of two bacterial HECT-like E3 ligases in complex with a human E2 reveal atomic details of pathogen-host interactions
[作者:Lin, DYW; Diao, JB; Chen, J,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1925-1930 , 文章类型: Article,,卷期:2012年109-6]
- In eukaryotes, ubiquitination is an important posttranslational process achieved through a cascade of ubiquitin-activating (E1), conjugating (E2), and ligase (E3) enzymes. Many pathogenic bacteria deliver virulence facto...
- Histone density is maintained during transcription mediated by the chromatin remodeler RSC and histone chaperone NAP1 in vitro
[作者:Kuryan, BG; Kim, J; Tran, NNH; Lombardo, SR; Venkatesh, S; Workman, JL; Carey, M,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1931-1936 , 文章类型: Article,,卷期:2012年109-6]
- ATPases and histone chaperones facilitate RNA polymerase II (pol II) elongation on chromatin. In vivo, the coordinated action of these enzymes is necessary to permit pol II passage through a nucleosome while restoring hi...
- On achieving high accuracy and reliability in the calculation of relative protein-ligand binding affinities
[作者:Wang, L; Berne, BJ; Friesner, RA,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1937-1942 , 文章类型: Article,,卷期:2012年109-6]
- We apply a free energy perturbation simulation method, free energy perturbation/replica exchange with solute tempering, to two modifications of protein-ligand complexes that lead to significant conformational changes, th...
- Subunit architecture of general transcription factor TFIIH
[作者:Gibbons, BJ; Brignole, EJ; Azubel, M; Murakami, K; Voss, NR; Bushnell, DA; Asturias, FJ; Kornberg, RD,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1949-1954 , 文章类型: Article,,卷期:2012年109-6]
- Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction. Consistency among all the struct...
- Functional characterization of the guanine nucleotide exchange factor (GEF) motif of GIV protein reveals a threshold effect in signaling
[作者:Garcia-Marcos, M; Kietrsunthorn, PS; Pavlova, Y; Adia, MA; Ghosh, P; Farquhar, MG,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1961-1966 , 文章类型: Article,,卷期:2012年109-6]
- Heterotrimeric G proteins are critical signal-transducing molecules controlled by a complex network of regulators. GIV (a.k.a. Girdin) is a unique component of this network and a nonreceptor guanine nucleotide exchange f...
- Role of histone deacetylases in transcription factor regulation and cell cycle modulation in endothelial cells in response to disturbed flow
[作者:Lee, DY; Lee, CI; Lin, TE; Lim, SH; Zhou, J; Tseng, YC; Chien, S; Chiu, JJ,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1967-1972 , 文章类型: Article,,卷期:2012年109-6]
- Vascular endothelial cells (ECs) are exposed to different flow patterns (i.e., disturbed vs. laminar), and the associated oscillatory shear stress (OSS) or pulsatile shear stress (PSS) lead to differential responses. We ...
- Active transcription and essential role of RNA polymerase II at the centromere during mitosis
[作者:Chan, FL; Marshall, OJ; Saffery, R; Kim, BW; Earle, E; Choo, KHA; Wong, LH,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1979-1984 , 文章类型: Article,,卷期:2012年109-6]
- Transcription of the centromeric regions has been reported to occur in G1 and S phase in different species. Here, we investigate whether transcription also occurs and plays a functional role at the mammalian centromere d...
- Thrombospondin-1 acts as a ligand for CD148 tyrosine phosphatase
[作者:Takahashi, K; Mernaugh, RL; Friedman, DB; Weller, R; Tsuboi, N; Yamashita, H; Quaranta, V; Takahashi, T,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1985-1990 , 文章类型: Article,,卷期:2012年109-6]
- CD148 is a receptor-type protein tyrosine phosphatase that is expressed in several cell types, including vascular endothelial cells and duct epithelial cells. Growing evidence demonstrates a prominent role for CD148 in n...
- Molecular architecture of the multisubunit homotypic fusion and vacuole protein sorting (HOPS) tethering complex
[作者:Brocker, C; Kuhlee, A; Gatsogiannis, C; Balderhaar, HJK; Honscher, C; Engelbrecht-Vandre, S; Ungermann, C; Raunser, S,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:1991-1996 , 文章类型: Article,,卷期:2012年109-6]
- Membrane fusion within the eukaryotic endomembrane system depends on the initial recognition of Rab GTPase on transport vesicles by multisubunit tethering complexes and subsequent coupling to SNARE-mediated fusion. The c...
- Class III PI3K Vps34 plays an essential role in autophagy and in heart and liver function
[作者:Jaber, N; Dou, ZX; Chen, JS; Catanzaro, J; Jiang, YP; Ballou, LM; Selinger, E; Ouyang, XS; Lin, RZ; Zhang, JH; Zong, WX,期刊:Proceedings of the National Academy of Sciences of the United States of America, 页码:2003-2008 , 文章类型: Article,,卷期:2012年109-6]
- A critical regulator of autophagy is the Class III PI3K Vps34 (also called PIK3C3). Although Vps34 is known to play an essential role in autophagy in yeast, its role in mammals remains elusive. To elucidate the physiolog...
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