- Unusual Activities of the Thioesterase Domain for the Biosynthesis of the Polycyclic Tetramate Macrolactam HSAF in Lysobacter enzymogenes C3
[作者:Lou, LL; Chen, HT; Cerny, RL; Li, YY; Shen, YM; Du, LC,期刊:Biochemistry, 页码:4-6 , 文章类型: Article,,卷期:2012年51-1]
- HSAF is an antifungal natural product with a new mode of action. A rare bacterial iterative PKS-NRPS assembles the HSAF skeleton. The biochemical characterization of the NRPS revealed that the thioesterase (TE) domain po...
- beta-Puromycin Selection of Modified Ribosomes for in Vitro Incorporation of beta-Amino Acids
[作者:Dedkova, LM; Fahmi, NE; Paul, R; del Rosario, M; Zhang, LQ; Chen, SX; Feder, G; Hecht, SM,期刊:Biochemistry, 页码:401-415 , 文章类型: Article,,卷期:2012年51-1]
- Ribosomally mediated protein biosynthesis is limited to alpha-L-amino acids. A strong bias against beta-L-amino acids precludes their incorporation into proteins in vivo and also in vitro in the presence of misacylated b...
- Phosphorylation of the p68 Subunit of Pol delta Acts as a Molecular Switch To Regulate Its Interaction with PCNA
[作者:Rahmeh, AA; Zhou, YJ; Xie, B; Li, H; Lee, EYC; Lee, MYWT,期刊:Biochemistry, 页码:416-424 , 文章类型: Article,,卷期:2012年51-1]
- DNA polymerase delta (Pol delta) is a central enzyme for eukaryotic DNA replication and repair. Pol delta is a complex of four subunits p125, p68, p50, and p12. The functional properties of Pol delta are largely determin...
- Sequence-Specific Base Pair Mimics Are Efficient Topoisomerase IB Inhibitors
[作者:Vekhoff, P; Duca, M; Guianvarc'h, D; Benhida, R; Arimondo, PB,期刊:Biochemistry, 页码:43-51 , 文章类型: Article,,卷期:2012年51-1]
- Topoisomerase IB controls DNA topology by cleaving DNA transiently. This property is used by inhibitors, such as camptothecin, that stabilize, by inhibiting the religation step, the cleavage complex, in which the enzyme ...
- Bacterial and Viral Sialidases: Contribution of the Conserved Active Site Glutamate to Catalysis
[作者:Chan, J; Watson, JN; Lu, A; Cerda, VC; Borgford, TJ; Bennet, AJ,期刊:Biochemistry, 页码:433-441 , 文章类型: Article,,卷期:2012年51-1]
- Mutagenesis of the conserved glutamic acid of influenza type A (E277) and Micromonospora viridifaciens (E260) sialidases was performed to probe the contribution of this strictly conserved residue to catalysis. Kinetic st...
- Initial- and Processive-Cut Products Reveal Cellobiohydrolase Rate Limitations and the Role of Companion Enzymes
[作者:Fox, JM; Levine, SE; Clark, DS; Blanch, HW,期刊:Biochemistry, 页码:442-452 , 文章类型: Article,,卷期:2012年51-1]
- Efforts to improve the activity of cellulases, which catalyze the hydrolysis of insoluble cellulose, have been hindered by uncertainty surrounding the mechanistic origins of rate-limiting phenomena and by an incomplete u...
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