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文章名     作者     期刊名     摘要     卷     期         如果没有找到您所需要的文献，请点击 ——此处申请     共58条记录   Revealing the Allosterome: Systematic Identification of Metabolite-Protein Interactions [作者：Orsak, T; Smith, TL; Eckert, D; Lindsley, JE; Borges, CR; Rutter, J,期刊：Biochemistry, 页码：225-232 , 文章类型： Article，,卷期：2012年51-1] Small molecule allostery modifies protein function but is not easily discovered. We introduce mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically (MIDAS), a method for ide... Probing Intein-Catalyzed Thioester Formation by Unnatural Amino Acid Substitutions in the Active Site [作者：Schwarzer, D; Ludwig, C; Thiel, IV; Mootz, HD,期刊：Biochemistry, 页码：233-242 , 文章类型： Article，,卷期：2012年51-1] Interns are single-turnover catalysts that splice themselves out of a precursor polypeptide chain. For most inteins, the first step of protein splicing is the formation of a thioester through an N-S acyl shift at the ups... Structural and Kinetic Isotope Effect Studies of Nicotinamidase (Pnc1) from Saccharomyces cerevisiae [作者：Smith, BC; Anderson, MA; Hoadley, KA; Keck, JL; Cleland, WW; Denu, JM,期刊：Biochemistry, 页码：243-256 , 文章类型： Article，,卷期：2012年51-1] Nicotinamidases catalyze the hydrolysis of nicotinamide to nicotinic acid and ammonia. Nicotinamidases are absent in mammals but function in NAD+ salvage in many bacteria, yeast, plants, protozoa, and metazoans. We have ... A Strongly Bound High-Spin Iron(II) Coordinates Cysteine and Homocysteine in Cysteine Dioxygenase [作者：Tchesnokov, EP; Wilbanks, SM; Jameson, GNL,期刊：Biochemistry, 页码：257-264 , 文章类型： Article，,卷期：2012年51-1] The first experimental evidence of a tight binding iron(II)-CDO complex is presented. These data enabled the relationship between iron bound and activity to be explicitly proven. Cysteine dioxygenase (CDO) from Rattus no... Flavin-Linked Erv-Family Sulfhydryl Oxidases Release Superoxide Anion during Catalytic Turnover [作者：Daithankar, VN; Wang, WZ; Trujillo, JR; Thorpe, C,期刊：Biochemistry, 页码：265-272 , 文章类型： Article，,卷期：2012年51-1] Typically, simple flavoprotein oxidases couple the oxidation of their substrates with the formation of hydrogen peroxide without release of significant levels of the superoxide ion. However, two evolutionarily related si... The Kinase Activity of the Helicobacter pylori Asp-tRNA(Asn)/Glu-tRNA(Gln) Amidotransferase Is Sensitive to Distal Mutations in Its Putative Ammonia Tunnel [作者：Zhao, LJ; Dewage, SW; Bell, MJ; Chang, KM; Fatma, S; Joshi, N; Silva, G; Cisneros, GA; Hendrickson, TL,期刊：Biochemistry, 页码：273-285 , 文章类型： Article，,卷期：2012年51-1] The Helicobacter pylori (Hp) Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase (AdT) plays important roles in indirect aminoacylation and translational fidelity. AdT has two active sites, in two separate subunits. Kinetic stu... The Crystal Structure of Human Endoplasmic Reticulum Aminopeptidase 2 Reveals the Atomic Basis for Distinct Roles in Antigen Processing [作者：Birtley, JR; Saridakis, E; Stratikos, E; Mavridis, IM,期刊：Biochemistry, 页码：286-295 , 文章类型： Article，,卷期：2012年51-1] Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 cooperate to trim a vast variety of antigenic peptide precursors to generate mature epitopes for binding to major histocompatibility class I molecules. We report here... Role of Glutamate 64 in the Activation of the Prodrug 5-Fluorocytosine by Yeast Cytosine Deaminase [作者：Wang, JF; Sklenak, S; Liu, AZ; Felczak, K; Wu, Y; Li, Y; Yan, HG,期刊：Biochemistry, 页码：475-486 , 文章类型： Article，,卷期：2012年51-1] Yeast cytosine deaminase (yCD) catalyzes the hydrolytic deamination of cytosine to uracil as well as the deamination of the prodrug 5-fluorocytosine (5FC) to the anticancer drug 5-fluorouracil. In this study, the role of... Human Chitotriosidase-Catalyzed Hydrolysis of Chitosan [作者：Eide, KB; Norberg, AL; Heggset, EB; Lindbom, AR; Varum, KM; Eijsink, VGH; Sorlie, M,期刊：Biochemistry, 页码：487-495 , 文章类型： Article，,卷期：2012年51-1] Chitotriosidase (HCHT) is one of two family 18 chitinases produced by humans, the other being acidic mammalian chitinase (AMCase). The enzyme is thought to be part of the human defense mechanism against fungal parasites,... In Vitro Selection and Characterization of a Novel Zn(II)-Dependent Phosphorothiolate Thiolesterase Ribozyme [作者：Wang, TP; Su, YC; Chen, Y; Liou, YM; Lin, KL; Wang, EC; Hwang, LC; Wang, YM; Chen, YH,期刊：Biochemistry, 页码：496-510 , 文章类型： Article，,卷期：2012年51-1] Here we present the in vitro selection of a novel ribozyme specific for Zn(2+)-dependent catalysis on hydrolysis of a phosphorothiolate thiolester bond. The ribozyme, called the TW17 ribozyme, was evolved and selected fr... Rapid Reaction Kinetics of Proline Dehydrogenase in the Multifunctional Proline Utilization A Protein [作者：Moxley, MA; Becker, DF,期刊：Biochemistry, 页码：511-520 , 文章类型： Article，,卷期：2012年51-1] The multifunctional proline utilization A (PutA) flavoenzyme from Escherichia coli catalyzes the oxidation of proline to glutamate in two reaction steps using separate proline dehydrogenase (PRODH) and Delta(1)-pyrroline... Trinucleotide Repeat DNA Alters Structure To Minimize the Thermodynamic Impact of 8-Oxo-7,8-dihydroguanine [作者：Volle, CB; Jarem, DA; Delaney, S,期刊：Biochemistry, 页码：52-62 , 文章类型： Article，,卷期：2012年51-1] In the phenomenon of trinucleotide repeat (TNR) expansion, an important interplay exists between DNA damage repair of 8-oxo-7,8-dihydroguanine (8-oxoG) and noncanonical structure formation. We show that TNR DNA adapts it... Identification of Formation of Initial Native Structure in Onconase from an Unfolded State [作者：Gahl, RF; Oswald, RE; Scheraga, HA,期刊：Biochemistry, 页码：521-532 , 文章类型： Article，,卷期：2012年51-1] In the oxidative folding of onconase, the stabilization of intermediates early in the folding process gives rise to efficient formation of its biologically active form. To identify the residues responsible for the initia... The Structure of the Thioredoxin-Triosephosphate Isomerase Complex Provides Insights into the Reversible Glutathione-Mediated Regulation of Triosephosphate Isomerase [作者：Hameed, MSS; Sarma, SP,期刊：Biochemistry, 页码：533-544 , 文章类型： Article，,卷期：2012年51-1] Protein-protein interactions are crucial for many biological functions. The redox interactome encompasses numerous weak transient interactions in which thioredoxin plays a central role. Proteomic studies have shown that ... Structure and Catalytic Mechanism of Nicotinate (Vitamin B(3)) Degradative Enzyme Maleamate Amidohydrolase from Bordetella bronchiseptica RB50 [作者：Kincaid, VA; Sullivan, ED; Klein, RD; Noel, JW; Rowlett, RS; Snidert, MJ,期刊：Biochemistry, 页码：545-554 , 文章类型： Article，,卷期：2012年51-1] The penultimate reaction in the oxidative degradation of nicotinate (vitamin B(3)) to fumarate in several species of aerobic bacteria is the hydrolytic deamination of maleamate to maleate, catalyzed by maleamate amidohyd... Catalysis-Based Inhibitors of the Calcium Signaling Function of CD38 [作者：Kwong, AKY; Chen, Z; Zhang, HM; Leung, FP; Lam, CMC; Ting, KY; Zhang, LR; Hao, Q; Zhang, LH; Lee, HC,期刊：Biochemistry, 页码：555-564 , 文章类型： Article，,卷期：2012年51-1] CD38 is a signaling enzyme responsible for catalyzing the synthesis of cyclic ADP ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate; both are universal Ca(2+) messenger molecules. Ablation of the CD38 gene...