- Structure of a Switchable Subtilisin Complexed with a Substrate and with the Activator Azide
[作者:Gallagher, T; Ruan, BA; London, M; Bryan, MA; Bryan, PN,期刊:Biochemistry, 页码:10389-10394 , 文章类型: Article,,卷期:2009年48-43]
- An engineered variant of the protease subtilisin from Bucillus amyloliquefaciens, in which the D32A mutation renders the enzyme's activity dependent on the presence of certain small anions such as fluoride or azide, has ...
- Analysis of the Structural and Functional Diversity of Plant Cell Wall Specific Family 6 Carbohydrate Binding Modules
[作者:Abbott, DW; Ficko-Blean, E; van Bueren, AL; Rogowski, A; Cartmell, A; Coutinho, PM; Henrissat, B; Gilbert, HJ; Boraston, AB,期刊:Biochemistry, 页码:10395-10404 , 文章类型: Article,,卷期:2009年48-43]
- Carbohydrate binding modules (CBMs) play important biological roles in targeting appended catalytic modules to their dedicated substrate(s) within complex macromolecular structures such as the plant cell wall. Because of...
- Surface Plasmon Resonance Binding Kinetics of Alzheimer's Disease Amyloid beta Peptide-Capturing and Plaque-Binding Monoclonal Antibodies
[作者:Ramakrishnan, M; Kandimalla, KK; Wengenack, TM; Howell, KG; Poduslo, JF,期刊:Biochemistry, 页码:10405-10415 , 文章类型: Article,,卷期:2009年48-43]
- Several different monoclonal antibodies (mAbs) have been actively developed in the field or Alzheimer's disease (AD) for basic science and clinical applications however, the binding kinetics of many of the mAbs with the ...
- Dramatic Differences in Organophosphorus Hydrolase Activity between Human and Chimeric Recombinant Mammalian Paraoxonase-1 Enzymes
[作者:Otto, TC; Harsch, CK; Yeung, DT; Magliery, TJ; Cerasoli, DM; Lenz, DE,期刊:Biochemistry, 页码:10416-10422 , 文章类型: Article,,卷期:2009年48-43]
- Human serum paraoxonase-1 (HuPON1) has the capacity to hydrolyze aryl esters, lactones, oxidized phospholipids, and organophosphorus (OP) compounds. HuPON1 and bacterially expressed chimeric recombinant PON1s (G2E6 and G...
- A Library of Fluorescent Peptides for Exploring the Substrate Specificities of Prolyl Isomerases
[作者:Zoldak, G; Aumuller, T; Lucke, C; Hritz, J; Ostenbrink, C; Fischer, G; Schmid, FX,期刊:Biochemistry, 页码:10423-10436 , 文章类型: Article,,卷期:2009年48-43]
- To fully explore the substrate specificities of prolyl isomerases, we synthesized a library of 20 tetrapeptides that are labeled with a 2-aminobenzoyl (Abz) group at the amino terminus and a p-nitroanilide (pNA) group at...
- The ATP-Dependent Amide Ligases DdaG and DdaF Assemble the Fumaramoyl-Dipeptide Scaffold of the Dapdiamide Antibiotics
[作者:Hollenhorst, MA; Clardy, J; Walsh, CT,期刊:Biochemistry, 页码:10467-10472 , 文章类型: Article,,卷期:2009年48-43]
- The enzymes DdaG and DdaF, encoded in the Pantoea agglomerans dapdiamide antibiotic biosynthetic gene cluster, when expressed in Escherichia coli, form the tandem amide bonds of the dapdiamide scaffold at the expense of ...
- Monomer-Dimer Equilibrium in Glutathione Transferases: A Critical Re-Examination
[作者:Fabrini, R; De Luca, A; Stella, L; Mei, G; Orioni, B; Ciccone, S; Federici, G; Lo Bello, M; Ricci, G,期刊:Biochemistry, 页码:10473-10482 , 文章类型: Article,,卷期:2009年48-43]
- Glutathione transferases (GSTs) are dimeric enzymes involved in cell detoxification versus many endogenous toxic compounds and xenobiotics. In addition, single monomers of GSTs appear to be involved in particular protein...
- Herpes Simplex Virus-1 Helicase-Primase: Roles of Each Subunit in DNA Binding and Phosphodiester Bond Formation
[作者:Cavanaugh, NA; Ramirez-Aguilar, KA; Urban, M; Kuchta, RD,期刊:Biochemistry, 页码:10199-10207 , 文章类型: Article,,卷期:2009年48-43]
- The helicase-primase complex from herpes simplex virus-1 contains three subunits, UL5, UL52, and UL8. We generated each of the potential two-subunit complexes, UL5-UL52, UL5-UL8, and UL52-UL8, and used a series of kineti...
- Solution Structure and Functional Characterization of Human Plasminogen Kringle 5
[作者:Battistel, MD; Grishaev, A; An, SSA; Castellino, FJ; Llinas, M,期刊:Biochemistry, 页码:10208-10219 , 文章类型: Article,,卷期:2009年48-43]
- The ligand binding properties of the kringle 5 (K5) domain of human plasminogen have been investigated via intrinsic tryptophan fluorescence. The oleic acid (OA) affinity for K5 was quantified, yielding an association co...
|