- DnaK Prevents Human Insulin Amyloid Fiber Formation on Hydrophobic Surfaces
[作者:Ballet, T; Brukert, F; Mangiagalli, P; Bureau, C; Boulange, L; Nault, L; Perret, T; Weidenhaupt, M,期刊:Biochemistry, 页码:2172-2180 , 文章类型: Article,,卷期:2012年51-11]
- We have developed a multiwell-based protein aggregation assay to study the kinetics of insulin adsorption and aggregation on hydrophobic surfaces and to investigate the molecular mechanisms involved. Protein-surface inte...
- Cystic Fibrosis Transmembrane Conductance Regulator: A Molecular Model Defines the Architecture of the Anion Conduction Path and Locates a "Bottleneck" in the Pore
[作者:Norimatsu, Y; Ivetac, A; Alexander, C; Kirkham, J; O'Donnell, N; Dawson, DC; Sansom, MSP,期刊:Biochemistry, 页码:2199-2212 , 文章类型: Article,,卷期:2012年51-11]
- We developed molecular models for the cystic fibrosis transmembrane conductance regulator chloride channel based on the prokaryotic ABC transporter, Sav1866. Here we analyze predicted pore geometry and side-chain orienta...
- In Vitro Phosphorylation of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase by Src Kinase
[作者:Cable, J; Prutzman, K; Gunawardena, HP; Schaller, MD; Chen, X; Campbell, SL,期刊:Biochemistry, 页码:2213-2223 , 文章类型: Article,,卷期:2012年51-11]
- Focal adhesion kinase (FAK), a key regulator of cell adhesion and migration, is overexpressed in many types of cancer. The C-terminal focal adhesion targeting (FAT) domain of FAK is necessary for proper localization of F...
- Calcium/Calmodulin Stimulates the Autophosphorylation of Elongation Factor 2 Kinase on Thr-348 and Ser-500 To Regulate Its Activity and Calcium Dependence
[作者:Tavares, CDJ; O'Brien, JP; Abramczyk, O; Devkota, AK; Shores, KS; Ferguson, SB; Kaoud, TS; Warthaka, M; Marshall, KD; Keller, KM; Zhang, Y; Brodbelt, JS; Ozpolat, B; Dolby, KN,期刊:Biochemistry, 页码:2232-2245 , 文章类型: Article,,卷期:2012年51-11]
- Eukaryotic elongation factor 2 kinase (eEF-2K) is an atypical protein kinase regulated by Ca2+ and calmodulin (CaM). Its only known substrate is eukaryotic elongation factor 2 (eEF-2), whose phosphorylation by eEF-2K imp...
- Structural Basis for Calcium and Phosphatidylserine Regulation of Phospholipase C delta 1
[作者:Lomasney, JW; Cheng, HF; Kobayashi, M; King, K,期刊:Biochemistry, 页码:2246-2257 , 文章类型: Article,,卷期:2012年51-11]
- Many membrane-associated enzymes, including those of the phospholipase C (PLC) superfamily, are regulated by specific interactions with lipids. Previously, we have shown that the C2 domain of PLC delta 1 is required for ...
- Distribution and Properties of the Genes Encoding the Biosynthesis of the Bacterial Cofactor, Pyrroloquinoline Quinone
[作者:Shen, YQ; Bonnot, F; Imsand, EM; RoseFigura, JM; Sjolander, K; Klinman, JP,期刊:Biochemistry, 页码:2265-2275 , 文章类型: Article,,卷期:2012年51-11]
- Pyrroloquinoline quinone (PQQ) is a small, redox active molecule that serves as a cofactor for several bacterial dehydrogenases, introducing pathways for carbon utilization that confer a growth advantage. Early studies h...
- Purification of Polyhydroxybutyrate Synthase from Its Native Organism, Ralstonia eutropha: Implications for the Initiation and Elongation of Polymer Formation in Vivo
[作者:Cho, M; Brigham, CJ; Sinskey, AJ; Stubbe, J,期刊:Biochemistry, 页码:2276-2288 , 文章类型: Article,,卷期:2012年51-11]
- Class I polyhydroxybutyrate (PHB) synthase (PhaC) from Ralstonia eutropha catalyzes the formation of PHB from (R)-3-hydroxybutyryl-CoA, ultimately resulting in the formation of insoluble granules. Previous mechanistic st...
- Removal of the C-Terminal Regulatory Domain of alpha-Isopropylmalate Synthase Disrupts Functional Substrate Binding
[作者:Huisman, FHA; Koon, N; Bulloch, EMM; Baker, HM; Baker, EN; Squire, CJ; Parker, EJ,期刊:Biochemistry, 页码:2289-2297 , 文章类型: Article,,卷期:2012年51-11]
- alpha-Isopropylmalate synthase (alpha-IPMS) catalyzes the metal-dependent aldol reaction between alpha-ketoisovalerate (alpha-KIV) and acetyl-coenzyme A (AcCoA) to give alpha-Isopropylmalate (alpha-IPM). This reaction is...
- OccK Channels from Pseudomonas aeruginosa Exhibit Diverse Single-Channel Electrical Signatures but Conserved Anion Selectivity
[作者:Liu, JM; Eren, E; Vijayaraghavan, J; Cheneke, BR; Indic, M; van den Berg, B; Movileanu, L,期刊:Biochemistry, 页码:2319-2330 , 文章类型: Article,,卷期:2012年51-11]
- Pseudomonas aeruginosa is a Gram-negative bacterium that utilizes substrate-specific outer membrane (OM) proteins for the uptake of small, water-soluble nutrients employed in the growth and function of the cell. In this ...
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