- Unravelling the Conformational Plasticity of the Extracellular Domain of a Prokaryotic nAChR Homologue in Solution by NMR
[作者:Chasapis, CT; Argyriou, AI; Corringer, PJ; Bentrop, D; Spyroulias, GA,期刊:Biochemistry, 页码:9681-9683 , 文章类型: Article,,卷期:2011年50-45]
- Pentameric ligand-gated ion channels (pLGICs) of the Cys loop family are transmembrane glycoproteins implicated in a variety of biological functions. Here, we present a solution NMR study of the extracellular domain of a...
- Lactose Permease and the Alternating Access Mechanism
[作者:Smirnova, I; Kasho, V; Kaback, HR,期刊:Biochemistry, 页码:9684-9693 , 文章类型: Article,,卷期:2011年50-45]
- Crystal structures of the lactose permease of Escherichia coli (LacY) reveal 12, mostly irregular transmembrane a-helices surrounding a large cavity open to the cytoplasm and a tightly sealed periplasmic side (inward-fac...
- Myotonic Dystrophy Type 1 RNA Crystal Structures Reveal Heterogeneous 1 x 1 Nucleotide UU Internal Loop Conformations
[作者:Kumar, A; Park, H; Fang, PF; Parkesh, R; Guo, M; Nettles, KW; Disney, MD,期刊:Biochemistry, 页码:9928-9935 , 文章类型: Article,,卷期:2011年50-45]
- RNA internal loops often display a variety of conformations in solution. Herein, we visualize conformational heterogeneity in the context of the 5'CUG/3'GUC repeat motif present in the RNA that causes myotonic dystrophy ...
- Activation and Inhibition of Pyruvate Carboxylase from Rhizobium etli
[作者:Zeczycki, TN; Menefee, AL; Jitrapakdee, S; Wallace, JC; Attwood, PV; St Maurice, M; Cleland, WW,期刊:Biochemistry, 页码:9694-9707 , 文章类型: Article,,卷期:2011年50-45]
- While crystallographic structures of the R. etli pyruvate carboxylase (PC) holoenzyme revealed the location and probable positioning of the essential activator, Mg(2+), and nonessential activator, acetyl-CoA, an understa...
- Interaction between the Biotin Carboxyl Carrier Domain and the Biotin Carboxylase Domain in Pyruvate Carboxylase from Rhizobium etli
[作者:Lietzan, AD; Menefee, AL; Zeczycki, TN; Kumar, S; Attwood, PV; Wallace, JC; Cleland, WW; St Maurice, M,期刊:Biochemistry, 页码:9708-9723 , 文章类型: Article,,卷期:2011年50-45]
- Pyruvate carboxylase (PC) catalyzes the ATP-dependent carboxylation of pyruvate to oxaloacetate, an important anaplerotic reaction in mammalian tissues. To effect catalysis, the tethered biotin of PC must gain access to ...
- Novel Insights into the Biotin Carboxylase Domain Reactions of Pyruvate Carboxylase from Rhizobium etli
[作者:Zeczycki, TN; Menefee, AL; Adina-Zada, A; Jitrapakdee, S; Surinya, KH; Wallace, JC; Attwood, PV; St Maurice, M; Cleland, WW,期刊:Biochemistry, 页码:9724-9737 , 文章类型: Article,,卷期:2011年50-45]
- The catalytic mechanism of the MgATP-dependent carboxylation of biotin in the biotin carboxylase domain of pyruvate carboxylase from R etli (RePC) is common to the biotin-dependent carboxylases. The current site-directed...
- Targeted Protein Degradation of Outer Membrane Decaheme Cytochrome MtrC Metal Reductase in Shewanella oneidensis MR-1 Measured Using Biarsenical Probe CrAsH-EDT(2)
[作者:Xiong, YJ; Chen, BW; Shi, L; Fredrickson, JK; Bigelow, DJ; Squier, TC,期刊:Biochemistry, 页码:9738-9751 , 文章类型: Article,,卷期:2011年50-45]
- Development of efficient microbial biofuel cells requires an ability to exploit interfacial electron transfer reactions to external electron acceptors, such as metal oxides; such reactions occur in the facultative anaero...
- Isolated Hb Providence beta 82Asn and beta 82Asp Fractions Are More Stable than Native HbA(0) under Oxidative Stress Conditions
[作者:Abraham, B; Hicks, W; Jia, YP; Baek, JH; Miller, JL; Alayash, AI,期刊:Biochemistry, 页码:9752-9766 , 文章类型: Article,,卷期:2011年50-45]
- We have previously shown that hydrogen peroxide (H(2)O(2)) triggers irreversible oxidation of amino acids exclusive to the beta-chains of purified human hemoglobin (HbAo). However, it is not clear, whether alpha- or beta...
- The Inactivating Factor of Glutamine Synthetase IF17 Is an Intrinsically Disordered Protein, Which Folds upon Binding to Its Target
[作者:Saelices, L; Galmozzi, CV; Florencio, FJ; Muro-Pastor, MI; Neira, JL,期刊:Biochemistry, 页码:9767-9778 , 文章类型: Article,,卷期:2011年50-45]
- In cyanobacteria, ammonium is incorporated into carbon skeletons by the sequential action of glutamine synthetase and glutamate synthase (GOGAT). The activity of Synechocystis sp. PCC 6803 glutamine synthetase type I (GS...
- Structural Transitions of Translation Initiation Factor IF2 upon GDPNP and GDP Binding in Solution
[作者:Rasmussen, LCV; Oliveira, CLP; Pedersen, JS; Sperling-Petersen, HU; Mortensen, KK,期刊:Biochemistry, 页码:9779-9787 , 文章类型: Article,,卷期:2011年50-45]
- Three protein factors ensure rapid and accurate initiation of translation in bacteria. Translation initiation factor IF2 is a ribosome-dependent GTPase, which is important for correct positioning of initiator tRNA on the...
- Properties of the Monomeric Form of Human 14-3-3 zeta Protein and Its Interaction with Tau and HspB6
[作者:Sluchanko, NN; Sudnitsyna, MV; Seit-Nebi, AS; Antson, AA; Gusev, NB,期刊:Biochemistry, 页码:9797-9808 , 文章类型: Article,,卷期:2011年50-45]
- Dimers formed by seven isoforms of the human 14-3-3 protein participate in multiple cellular processes. The dimeric form has been extensively characterized; however, little is known about the structure and properties of ...
- Assessment of a Fully Active Class A G Protein-Coupled Receptor Isolated from in Vitro Folding
[作者:Bosse, M; Thomas, L; Hassert, R; Beck-Sickinger, AG; Huster, D; Schmidt, P,期刊:Biochemistry, 页码:9817-9825 , 文章类型: Article,,卷期:2011年50-45]
- We provide a protocol for the preparation of fully active Y2 G protein-coupled receptors (GPCRs). Although a valuable target for pharmaceutical research, information about the structure and dynamics of these molecules re...
|