- The D1-D61N Mutation in Synechocystis sp PCC 6803 Allows the Observation of pH-Sensitive Intermediates in the Formation and Release of O-2 from Photosystem II
[作者:Dilbeck, PL; Hwang, HJ; Zaharieva, I; Gerencser, L; Dau, H; Burnap, RL,期刊:Biochemistry, 页码:1079-1091 , 文章类型: Article,,卷期:2012年51-6]
- The active site of photosynthetic water oxidation by Photosystem II (PSII) is a manganese-calcium cluster (Mn4CaO5). A postulated catalytic base is assumed to be crucial. CP43-Arg357, which is a candidate for the identit...
- The Membrane Modulates Internal Proton Transfer in Cytochrome c Oxidase
[作者:Ojemyr, LN; von Ballmoos, C; Faxen, K; Svahn, E; Brzezinski, P,期刊:Biochemistry, 页码:1092-1100 , 文章类型: Article,,卷期:2012年51-6]
- The functionality of membrane proteins is often modulated by the surrounding membrane. Here, we investigated the effect of membrane reconstitution of purified cytochrome c oxidase (CytcO) on the kinetics and thermodynami...
- Crystallographic Analysis of Active Site Contributions to Regiospecificity in the Diiron Enzyme Toluene 4-Monooxygenase
[作者:Bailey, LJ; Acheson, JF; McCoy, JG; Elsen, NL; Phillips, GN; Fox, BG,期刊:Biochemistry, 页码:1101-1113 , 文章类型: Article,,卷期:2012年51-6]
- Crystal structures of toluene 4-monooxygenase hydroxylase in complex with reaction products and effector protein reveal active site interactions leading to regiospecificity. Complexes with phenolic products yield an asym...
- Protein-Bound Water Molecules in Primate Red- and Green-Sensitive Visual Pigments
[作者:Katayama, K; Furutani, Y; Imai, H; Kandori, H,期刊:Biochemistry, 页码:1126-1133 , 文章类型: Article,,卷期:2012年51-6]
- Protein-bound water molecules play crucial roles in the structure and function of proteins. The functional role of water molecules has been discussed for rhodopsin, the light sensor for twilight vision, on the basis of X...
- A Processive Carbohydrate Polymerase That Mediates Bifunctional Catalysis Using a Single Active Site
[作者:May, JF; Levengood, MR; Splain, RA; Brown, CD; Kiessling, LL,期刊:Biochemistry, 页码:1148-1159 , 文章类型: Article,,卷期:2012年51-6]
- Even in the absence of a template, glycosyltransferases can catalyze the synthesis of carbohydrate polymers of specific sequence. The paradigm has been that one enzyme catalyzes the formation of one type of glycosidic li...
- Structure of Mandelate Racemase with Bound Intermediate Analogues Benzohydroxamate and Cupferron
[作者:Lietzan, AD; Nagar, M; Pellmann, EA; Bourque, JR; Bearne, SL; St Maurice, M,期刊:Biochemistry, 页码:1160-1170 , 文章类型: Article,,卷期:2012年51-6]
- Mandelate racemase (MR, EC 5.1.2.2) from Pseudomonas putida catalyzes the Mg2+-dependent interconversion of the enantiomers of mandelate, stabilizing the altered substrate in the transition state by 26 kcal/mol relative ...
- ArsH from the Cyanobacterium Synechocystis sp PCC 6803 Is an Efficient NADPH-Dependent Quinone Reductase
[作者:Hervas, M; Lopez-Maury, L; Leon, P; Sanchez-Riego, AM; Florencio, FJ; Navarro, JA,期刊:Biochemistry, 页码:1178-1187 , 文章类型: Article,,卷期:2012年51-6]
- The cyanobacterium Synechocystis sp. PCC 6803 possesses an arsenic resistance operon that encodes, among others, an ArsH protein. ArsH is a flavin mononucleotide (FMN)-containing protein of unknown function and a member ...
- Two ZnF-UBP Domains in Isopeptidase T (USP5)
[作者:Avvakumov, GV; Walker, JR; Xue, S; Allali-Hassani, A; Asinas, A; Nair, UB; Fang, XY; Zuo, XB; Wane, YX; Wilkinson, KD; Dhe-Paganon, S,期刊:Biochemistry, 页码:1188-1198 , 文章类型: Article,,卷期:2012年51-6]
- Human ubiquitin-specific cysteine protease 5 (USP5, also known as ISOT and isopeptidase T), an 835-residue multidomain enzyme, recycles ubiquitin by hydrolyzing isopeptide bonds in a variety of unanchored polyubiquitin s...
- Probing the Donor and Acceptor Substrate Specificity of the gamma-Glutamyl Transpeptidase
[作者:Hu, X; Legler, PM; Khavrutskii, I; Scorpio, A; Compton, JR; Robertson, KL; Friedlander, AM; Wallqvist, A,期刊:Biochemistry, 页码:1199-1212 , 文章类型: Article,,卷期:2012年51-6]
- gamma-Glutamyl transpeptidase (GGT) is a two-substrate enzyme that plays a central role in glutathione metabolism and is a potential target for drug design. GGT catalyzes the cleavage of gamma-glutamyl donor substrates a...
- Structural Studies of the Spinosyn Rhamnosyltransferase, SpnG
[作者:Isiorho, EA; Liu, HW; Keatinge-Clay, AT,期刊:Biochemistry, 页码:1213-1222 , 文章类型: Article,,卷期:2012年51-6]
- Spinosyns A and D (spinosad), like many other complex polyketides, are tailored near the end of their biosyntheses through the addition of sugars. SpnG, which catalyzes their 9-OH rhamnosylation, is also capable of addin...
- Domain Structure and Denaturation of a Dimeric Mip-like Peptidyl-Prolyl cis-trans Isomerase from Escherichia coli
[作者:Jana, B; Bandhu, A; Mondal, R; Biswas, A; Sau, K; Sau, S,期刊:Biochemistry, 页码:1223-1237 , 文章类型: Article,,卷期:2012年51-6]
- FKBP22, a protein expressed by Escherichia coli, possesses PPIase (peptidyl-prolyl cis-trans isomerase) activity, binds FK506 (an immunosuppressive drug), and shares homology with Legionella Mip (a virulence factor) and ...
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