- In a Head-on Collision, Two RNA Polymerases Approaching One Another on the Same DNA May Pass by One Another
[作者:Ma, N; McAllister, WT,期刊:Journal of Molecular Biology, 页码:808-812 , 文章类型: Article,,卷期:2009年391-5]
- Using a template that contains promoters for T3 and T7 RNA polymerases, (RNAPs) in opposing orientations, and His-tagged derivatives of these RNAPs that allow immobilization on solid matrices, we have determined that a T...
- Direct Optical Microscopic Observation of the Microtubule Polymerization Intermediate Sheet Structure in the Presence of Gas7
[作者:Uchida, T; Akiyama, H; Sakamoto, W; Koga, T; Yan, K; Uchida, C; Hirose, K; Itoh, TJ,期刊:Journal of Molecular Biology, 页码:849-857 , 文章类型: Article,,卷期:2009年391-5]
- The process of microtubule elongation is thought to consist of two stages-formation of a tubulin sheet structure and its closure into a tube. However, real-time observation of this process has been difficult. Here, by ut...
- High-yield Heterologous Expression of Wild Type and Mutant Ca2+ ATPase: Characterization of Ca2+ Binding Sites by Charge Transfer
[作者:Liu, YY; Pilankatta, R; Lewis, D; Inesi, G; Tadini-Buoninsegni, F; Bartolommei, G; Moncelli, MR,期刊:Journal of Molecular Biology, 页码:858-871 , 文章类型: Article,,卷期:2009年391-5]
- High-yield heterologous SERCA1 (Ca2+ ATPase) expression was obtained in COS-1 cells infected with recombinant adenovirus vector (rAdSERCA). Higher transcription and expression were obtained in the presence of a His(6) ta...
- Deciphering Membrane Insertion of the Diphtheria Toxin T Domain by Specular Neutron Reflectometry and Solid-State NMR Spectroscopy
[作者:Chenal, A; Prongidi-Fix, L; Perier, A; Aisenbrey, C; Vernier, G; Lambotte, S; Fragneto, G; Bechinger, B; Gillet, D; Forge, V; Ferrand, M,期刊:Journal of Molecular Biology, 页码:872-883 , 文章类型: Article,,卷期:2009年391-5]
- Insertion and translocation of soluble proteins into and across biological membranes are involved in many physiological and pathological processes, but remain poorly understood. Here, we describe the pH-dependent membran...
- Structural and Functional Divergence within the Dim1/KsgA Family of rRNA Methyltransferases
[作者:Pulicherla, N; Pogorzala, LA; Xu, ZL; O'Farrell, HC; Musayev, FN; Scarsdale, JN; Sia, EA; Culver, GM; Rife, JP,期刊:Journal of Molecular Biology, 页码:884-893 , 文章类型: Article,,卷期:2009年391-5]
- The enzymes of the KsgA/Dim1 family are universally distributed throughout all phylogeny; however, structural and functional differences are known to exist. The well-characterized function of these enzymes is to dimethyl...
- Distinct Structural Features of Cyclothiazide Are Responsible for Effects on Peak Current Amplitude and Desensitization Kinetics at iGluR2
[作者:Hald, H; Ahring, PK; Timmermann, DB; Liljefors, T; Gajhede, M; Kastrup, JS,期刊:Journal of Molecular Biology, 页码:906-917 , 文章类型: Article,,卷期:2009年391-5]
- Ionotropic glutamate receptors (iGluRs) mediate fast excitatory neurotransmission. Upon glutamate application, 2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl)propionic acid receptors undergo rapid and almost complete desensi...
- Dimer Formation of a Stabilized G beta 1 Variant: A Structural and Energetic Analysis
[作者:Thoms, S; Max, KEA; Wunderlich, M; Jacso, T; Lilie, H; Reif, B; Heinemann, U; Schmid, FX,期刊:Journal of Molecular Biology, 页码:918-932 , 文章类型: Article,,卷期:2009年391-5]
- In previous work, a strongly stabilized variant of the beta 1 domain of streptococcal protein G (G beta 1) was obtained by an in vitro selection method. This variant, termed G beta 1-M2, contains the four substitutions E...
- Modulation of Calmodulin Plasticity by the Effect of Macromolecular Crowding
[作者:Homouz, D; Sanabria, H; Waxham, MN; Cheung, MS,期刊:Journal of Molecular Biology, 页码:933-943 , 文章类型: Article,,卷期:2009年391-5]
- In vitro biochemical reactions are most often studied in dilute solution, a poor mimic of the intracellular space of eukaryotic cells, which are crowded with mobile and immobile macromolecules. Such crowded conditions ex...
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