- Variations in Protein-Flavin Hydrogen Bonding in a Light, Oxygen, Voltage Domain Produce Non-Arrhenius Kinetics of Adduct Decay
[作者:Zoltowski, BD; Nash, AI; Gardner, KH,期刊:Biochemistry, 页码:8771-8779 , 文章类型: Article,,卷期:2011年50-41]
- Light, oxygen, voltage (LOV) domains utilize a conserved blue light-dependent mechanism to control a diverse array of effector domains in biological and engineered proteins. Variations in the kinetics and efficiency of L...
- Interaction between the D2 Dopamine Receptor and Neuronal Calcium Sensor-1 Analyzed by Fluorescence Anisotropy
[作者:Woll, MP; De Cotiis, DA; Bewley, MC; Tacelosky, DM; Levenson, R; Flanagan, JM,期刊:Biochemistry, 页码:8780-8791 , 文章类型: Article,,卷期:2011年50-41]
- Neuronal calcium sensor-1 (NCS-1) is a small calcium binding protein that plays a key role in the internalization and desensitization of activated D2 dopamine receptors (D2Rs). Here, we have used fluorescence anisotropy ...
- The Second Ca(2+)-Binding Domain of NCX1 Binds Mg(2+) with High Affinity
[作者:Breukels, V; Konijnenberg, A; Nabuurs, SM; Touw, WG; Vuister, GW,期刊:Biochemistry, 页码:8804-8812 , 文章类型: Article,,卷期:2011年50-41]
- We report the effects of binding of Mg(2+) to the second Ca(2+)-binding domain (CBD2) of the sodium calcium exchanger. CBD2 is known to bind two Ca(2+) ions using its Ca(2+)-binding sites I and II. Here, we show by nucle...
- Structural Studies of the Molybdenum Center of Mitochondrial Amidoxime Reducing Component (mARC) by Pulsed EPR Spectroscopy and (17)O-Labeling
[作者:Rajapakshe, A; Astashkin, AV; Klein, EL; Reichmann, D; Mendel, RR; Bittner, F; Enemark, JH,期刊:Biochemistry, 页码:8813-8822 , 文章类型: Article,,卷期:2011年50-41]
- Mitochondrial amidoxime reducing components (mARC-1 and mARC-2) represent a novel group of Mo-containing enzymes in eukaryotes. These proteins form the catalytic part of a three-component enzyme complex known to be respo...
- Influence of Substrate Modification and C-Terminal Truncation on the Active Site Structure of Substrate-Bound Heme Oxygenase from Neisseriae meningitidis. A (1)H NMR Study
[作者:Peng, DG; Satterlee, JD; Ma, LH; Dallas, JL; Smith, KM; Zhang, XH; Sato, M; La Mar, GN,期刊:Biochemistry, 页码:8823-8833 , 文章类型: Article,,卷期:2011年50-41]
- Heine oxygenase (HO), from the pathogenic bacterium N. meningitidis (NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits some key differences. The crystal structure appears more co...
- Hexose Transporter GLUT1 Harbors Several Distinct Regulatory Binding Sites for Flavones and Tyrphostins
[作者:Perez, A; Ojeda, P; Ojeda, L; Salas, M; Rivas, CI; Vera, JC; Reyes, AM,期刊:Biochemistry, 页码:8834-8845 , 文章类型: Article,,卷期:2011年50-41]
- The facilitative hexose transporter GLUT1 activity is blocked by tyrosine kinase inhibitors that include natural products such as flavones and isoflavones and synthetic compounds such as tyrphostins, molecules that are s...
- Redefining the Minimal Substrate Tolerance of Mande late Racemase. Racemization of Trifluorolactate
[作者:Nagar, M; Narmandakh, A; Khalak, Y; Bearne, SL,期刊:Biochemistry, 页码:8846-8852 , 文章类型: Article,,卷期:2011年50-41]
- Mande late racemase (EC 5.1.2.2) from Pseudomonas putida catalyzes the interconversion of the enantiomers of mandelic acid and a variety of aryl- and heteroaryl-substituted mandelate derivatives, suggesting that beta,gam...
- Amino Acid Determinants of Substrate Selectivity in the Trypanosoma brucei Sphingolipid Synthase Family
[作者:Goren, MA; Fox, BG; Bangs, JD,期刊:Biochemistry, 页码:8853-8861 , 文章类型: Article,,卷期:2011年50-41]
- The substrate selectivity of four Trypanosoma brucei sphingolipid syntheses was examined. TbSLS1, an inositol phosphorylceramide (IPC) synthase, and TbSIS4, a bifunctional sphingomyelin (SM)/ethanolamine phosphorylcerami...
- Structural Insights into the Activation and Inhibition of Histo-Aspartic Protease from Plasmodium falciparum
[作者:Bhaumik, P; Xiao, HG; Hidaka, K; Gustchina, A; Kiso, Y; Yada, RY; Wlodawer, A,期刊:Biochemistry, 页码:8862-8879 , 文章类型: Article,,卷期:2011年50-41]
- Histo-aspartic protease (HAP) from Plasmodium falciparum is a promising target for the development of novel antimalarial drugs. The sequence of HAP is highly similar to those of pepsin-like aspartic proteases, but one of...
- Photochemistry of Acetabularia Rhodopsin II from a Marine Plant, Acetabularia acetabulum
[作者:Kikukawa, T; Shimono, K; Tamogami, J; Miyauchi, S; Kim, SY; Kimura-Someya, T; Shirouzu, M; Jung, KH; Yokoyama, S; Kamo, N,期刊:Biochemistry, 页码:8888-8898 , 文章类型: Article,,卷期:2011年50-41]
- Acetabularia rhodopsins are the first microbial rhodopsins discovered in a marine plant organism, Acetabularia acetabulum. Previously, we expressed Acetabularia rhodopsin II (ARII) by a cell-free system from one of two o...
- Substrate Specificity of Sphingobium chlorophenolicum 2,6-Dichlorohydroquinone 1,2-Dioxygenase
[作者:Machonkin, TE; Doerner, AE,期刊:Biochemistry, 页码:8899-8913 , 文章类型: Article,,卷期:2011年50-41]
- PcpA is an aromatic ring-cleaving dioxygenase that is homologous to the well-characterized Fe(II)-dependent catechol, extradiol dioxygenases. This enzyme catalyzes the oxidative cleavage of 2,6-dichlorohydroquinone in th...
- Identification, Tissue Distribution, and Molecular Modeling of Novel Human Isoforms of the Key Enzyme in Sialic Acid Synthesis, UDP-GIcNAc 2-Epimerase/ManNAc Kinase
[作者:Yardeni, T; Choekyi, T; Jacobs, K; Ciccone, C; Patzel, K; Anikster, Y; Gahl, WA; Kurochkina, N; Huizing, M,期刊:Biochemistry, 页码:8914-8925 , 文章类型: Article,,卷期:2011年50-41]
- UDP-GIcNAc 2-epimerase/ManNAc kinase (GNE) catalyzes the first two committed steps in sialic acid synthesis. In addition to the three previously described human GNE isoforms (hGNE1-hGNE3), our database and polymerase cha...
- Divergence of Structure and Function in the Haloacid Dehalogenase Enzyme Superfamily: Bacteroides thetaiotaomicron BT2127 Is an Inorganic Pyrophosphatase
[作者:Huang, H; Patskovsky, Y; Toro, R; Farelli, JD; Pandya, C; Almo, SC; Allen, KN; Dunaway-Mariano, D,期刊:Biochemistry, 页码:8937-8949 , 文章类型: Article,,卷期:2011年50-41]
- The explosion of protein sequence information requires that current strategies for function assignment evolve to complement experimental approaches with computationally based function prediction. This necessitates the de...
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