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文章名     作者     期刊名     摘要     卷     期         如果没有找到您所需要的文献，请点击 ——此处申请     共23条记录   Design of Native-like Proteins through an Exposure-Dependent Environment Potential [作者：DeLuca, S; Dorr, B; Meiler, J,期刊：Biochemistry, 页码：8521-8528 , 文章类型： Article，,卷期：2011年50-40] We hypothesize that the degree of surface exposure of amino acid side chains within a globular, soluble protein has been optimized in evolution, not only to minimize the solvation free energy of the monomeric protein but... Mapping Unstructured Regions and Synergistic Folding in Intrinsically Disordered Proteins with Amide H/D Exchange Mass Spectrometry [作者：Keppel, TR; Howard, BA; Weis, DD,期刊：Biochemistry, 页码：8722-8732 , 文章类型： Article，,卷期：2011年50-40] Mapping the structured and disordered regions and identifying disorder-to-order transitions are essential to understanding intrinsically disordered proteins (IDPs). One technique that can provide such information is H/D ... Structure-Function Analysis from the Outside In: Long-Range Tertiary Contacts in RNA Exhibit Distinct Catalytic Roles [作者：Benz-Moy, TL; Herschag, D,期刊：Biochemistry, 页码：8733-8755 , 文章类型： Article，,卷期：2011年50-40] The conserved catalytic core of the Tetrahymena group I ribozyme is encircled by peripheral elements. We have conducted a detailed structure-function study of the five long-range tertiary contacts that fasten these dista... Complexity Generation in Fungal Peptidyl Alkaloid Biosynthesis: Oxidation of Fumiquinazoline A to the Heptacyclic Hemiaminal Fumiquinazoline C by the Flavoenzyme Af12070 from Aspergillus fumigatus [作者：Ames, BD; Haynes, SW; Gao, X; Evans, BS; Kelleher, NL; Tang, Y; Walsh, CT,期刊：Biochemistry, 页码：8756-8769 , 文章类型： Article，,卷期：2011年50-40] The human pathogen Aspergillus fumigatus makes a series of fumiquinazoline (FQ) peptidyl alkaloids of increasing scaffold complexity using L-Trp, 2 equiv of L-Ala, and the non-proteinogenic amino acid anthranilate as bui... The Respiratory Syncytial Virus Transcription Antiterminator M(2-1) Is a Highly Stable, Zinc Binding Tetramer with Strong pH-Dependent Dissociation and a Monomeric Unfolding Intermediate [作者：Esperante, SA; Chemes, LB; Sanchez, IE; de Prat-Gay, G,期刊：Biochemistry, 页码：8529-8539 , 文章类型： Article，,卷期：2011年50-40] The human respiratory syncytial virus M(2-1) transcription antiterminator is an essential elongation factor required by the RNA polymerase for effective transcription beyond the first two nonstructural genes. Its exclusi... Effects of a Protecting Osmolyte on the Ion Atmosphere Surrounding DNA Duplexes [作者：Blose, JM; Pabit, SA; Meisburger, SP; Li, L; Jones, CD; Pollack, L,期刊：Biochemistry, 页码：8540-8547 , 文章类型： Article，,卷期：2011年50-40] Osmolytes are small, chemically diverse, organic solutes that function as an essential component of cellular stress response. Protecting osmolytes enhance protein stability via preferential exclusion, and nonprotecting o... Thermodynamic Analysis of Self-Assembly in Coiled-Coil Biomaterials [作者：Tsang, BP; Bretscher, HS; Kokona, B; Manning, RS; Fairman, R,期刊：Biochemistry, 页码：8548-8558 , 文章类型： Article，,卷期：2011年50-40] Coiled-coil protein structural motifs have proven amenable to the design of structurally well-defined biomaterials. Mesoscale structural properties can be fairly well predicted based on rules governing the chemical inter... Breaking the Proximal Fe(II)-N(His) Bond in Heme Proteins through Local Structural Tension: Lessons from the Heme b Proteins Nitrophorin 4, Nitrophorin 7, and Related Site-Directed Mutant Proteins [作者：He, CM; Neya, S; Knipp, M,期刊：Biochemistry, 页码：8559-8575 , 文章类型： Article，,卷期：2011年50-40] The factors leading to the breakage of the proximal iron-histidine bond in the ferroheme protein soluble guanylate cyclase (sGC) are still a matter of debate. This event is a key mechanism in the sensing of NO that leads... The Physical Basis of FGFR3 Response to fgf1 and fgf2 [作者：Chen, FH; Hristova, K,期刊：Biochemistry, 页码：8576-8582 , 文章类型： Article，,卷期：2011年50-40] Fibroblast growth factors (fgfs) play important roles in embryonic development and in adult life by controlling cell proliferation, differentiation, and migration. There are 18 known fgfs which activate four fibroblast g... Substitutions of Coenzyme-Binding, Nonpolar Residues Improve the Low-Temperature Activity of Thermophilic Dehydrogenases [作者：Hayashi, S; Akanuma, S; Onuki, W; Tokunaga, C; Yamagishi, A,期刊：Biochemistry, 页码：8583-8593 , 文章类型： Article，,卷期：2011年50-40] Although enzymes of thermophilic organisms are often very resistant to thermal denaturation, they are usually less active than their mesophilic or psychrophilic homologues at moderate or low temperatures. To explore the ... An N-Terminal Protein Degradation Tag Enables Robust Selection of Highly Active Enzymes [作者：Butz, M; Neuenschwander, M; Kast, P; Hilvert, D,期刊：Biochemistry, 页码：8594-8602 , 文章类型： Article，,卷期：2011年50-40] Degradation tags are short peptide sequences that target proteins for destruction by housekeeping proteases. We previously utilized the C-terminal SsrA tag in directed evolution experiments to decrease the intracellular ... Structure and Mechanism of PhnP, a Phosphodiesterase of the Carbon-Phosphorus Lyase Pathway [作者：He, SM; Wathier, M; Podzelinska, K; Wong, M; McSorley, FR; Asfaw, A; Hove-Jensen, B; Jia, ZC; Zechel, DL,期刊：Biochemistry, 页码：8603-8615 , 文章类型： Article，,卷期：2011年50-40] PhnP is a phosphodiesterase that plays an important role within the bacterial carbon-phosphorus lyase (CP-lyase) pathway by recycling a "dead-end" intermediate, 5-phospho-alpha-D-ribosyl 1,2-cyclic phosphate, that is for... Structural and Mechanistic Analysis of a Novel Class of Shikimate Dehydrogenases: Evidence for a Conserved Catalytic Mechanism in the Shikimate Dehydrogenase Family [作者：Peek, J; Lee, J; Hu, S; Senisterra, G; Christendat, D,期刊：Biochemistry, 页码：8616-8627 , 文章类型： Article，,卷期：2011年50-40] Shikimate dehydrogenase (SDH) catalyzes the reversible NADPH-dependent reduction of 3-dehydroshikimate to shikimate. This reaction represents the fourth step of the shikimate pathway, the essential route for the biosynth... Simultaneous Stereoinversion and Isomerization at the Asp-4 Residue in beta B2-Crystallin from the Aged Human Eye Lenses [作者：Fujii, N; Kawaguchi, T; Sasaki, H; Fujii, N,期刊：Biochemistry, 页码：8628-8635 , 文章类型： Article，,卷期：2011年50-40] The lens proteins are composed of alpha-, beta-, and gamma-crystallins that interact with each other to maintain the transparency and refractive power of the lens. Because the lens crystallins are long-lived proteins, th... Design, Overexpression, and Purification of Polymerization-Blocked Yeast alpha beta-Tubulin Mutants [作者：Johnson, V; Ayaz, P; Huddleston, P; Rice, LM,期刊：Biochemistry, 页码：8636-8644 , 文章类型： Article，,卷期：2011年50-40] Microtubule dynamics play essential roles in intracellular organization and cell division. They result from structural and biochemical properties of alpha beta-tubulin heterodimers and how these polymerizing subunits int... Probing the Structural and Energetic Basis of Kinesin-Microtubule Binding Using Computational Alanine-Scanning Mutagenesis [作者：Li, MH; Zheng, WJ,期刊：Biochemistry, 页码：8645-8655 , 文章类型： Article，,卷期：2011年50-40] Kinesin microtubule (MT) binding plays a critical role in facilitating and regulating the motor function of kinesins. To obtain a detailed structural and energetic picture of kenesin-MT binding, we performed large-scale ...