- Glutamate-haem ester bond formation is disfavoured in flavocytochrome P450 BM3: characterization of glutamate substitution mutants at the haem site of P450 BM3
[作者:Girvan, HM; Levy, CW; Williams, P; Fisher, K; Cheesman, MR; Rigby, SEJ; Leys, D; Munro, AW,期刊:Biochemical Journal, 页码:455-466 , 文章类型: Article,,卷期:2010年427-Part 3]
- Bacillus megaterium flavocytochrorne P450 BM3 (CYP102A1) is a biotechnologically important cytochrome P450/P450 reductase fusion enzyme. Mutants I401E, F261E and L86E were engineered near the haem 5-methyl group, to expl...
- Transglutaminase-2: a new endostatin partner in the extracellular matrix of endothelial cells
[作者:Faye, C; Inforzato, A; Bignon, M; Hartmann, DJ; Muller, L; Ballut, L; Olsen, BR; Day, AJ; Ricard-Blum, S,期刊:Biochemical Journal, 页码:467-475 , 文章类型: Article,,卷期:2010年427-Part 3]
- Endostatin, a C-terminal fragment of collagen XVIII, binds to TG-2 (transglutaminase-2) in a cation-dependent manner. Recombinant human endostatin binds to TG-2 with an affinity in the nanomolar range (K-d = 6.8 nM). Enz...
- Lipopolysaccharide interaction is decisive for the activity of the antimicrobial peptide NK-2 against Escherichia coil and Proteus mirabilis
[作者:Hammer, MU; Brauser, A; Olak, C; Brezesinski, G; Goldmann, T; Gutsmann, T; Andra, J,期刊:Biochemical Journal, 页码:477-488 , 文章类型: Article,,卷期:2010年427-Part 3]
- Phosphatidylglycerol is a widely used mimetic to study the effects of AMPs (antimicrobial peptides) on the bacterial cytoplasmic membrane. However, the antibacterial activities of novel NK-2-derived AMPs could not be suf...
- The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1 delta/epsilon
[作者:Sugiyama, Y; Hatano, N; Sueyoshi, N; Suetake, I; Tajima, S; Kinoshita, E; Kinoshita-Kikuta, E; Koike, T; Kameshita, I,期刊:Biochemical Journal, 页码:489-497 , 文章类型: Article,,卷期:2010年427-Part 3]
- Dnmt1 (DNA methyltansferase 1) is an enzyme that recognizes and methylates hemimethylated DNA during DNA replication to maintain methylation patterns. The N-terminal region of Dnmt1 is known to form an independent domain...
- Signal peptide peptidase (SPP) assembles with substrates and misfolded membrane proteins into distinct oligomeric complexes
[作者:Schrul, B; Kapp, K; Sinning, I; Dobberstein, B,期刊:Biochemical Journal, 页码:523-534 , 文章类型: Article,,卷期:2010年427-Part 3]
- SPP (signal peptide peptidase) is an aspartyl intramembrane cleaving protease, which processes a subset of signal peptides, and is linked to the quality control of ER (endoplasmic reticulum) membrane proteins. We analyse...
- Proteolytic processing of an Arabidopsis membrane-bound NAC transcription factor is triggered by cold-induced changes in membrane fluidity
[作者:Seo, PJ; Kim, MJ; Song, JS; Kim, YS; Kim, HJ; Park, CM,期刊:Biochemical Journal, 页码:359-367 , 文章类型: Article,,卷期:2010年427-Part 3]
- Changes in membrane fluidity are the earliest cellular events that occur in plant cells upon exposure to cold. This subsequently triggers physiological processes, such as calcium influx and reorganization of actin cytosk...
- Genomic organization and regulation of the human orexin (hypocretin) receptor 2 gene: identification of alternative promoters
[作者:Chen, J; Randeva, HS,期刊:Biochemical Journal, 页码:377-390 , 文章类型: Article,,卷期:2010年427-Part 3]
- Orexins (hypocretins), acting via their receptors, are involved in the control of feeding behaviour, sleep, arousal and energy homoeostasis. However, regulation of the human orexin receptor 2 (hOX2R) gene remains unknown...
- Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus
[作者:Shiryaev, SA; Radichev, IA; Ratnikov, BI; Aleshin, AE; Gawlik, K; Stec, B; Frisch, C; Knappik, A; Strongin, AY,期刊:Biochemical Journal, 页码:369-376 , 文章类型: Article,,卷期:2010年427-Part 3]
- There is a need to develop inhibitors of mosquito-borne flaviviruses, including WNV (West Nile virus). In the present paper, we describe a novel and efficient recombinant-antibody technology that led us to the isolation ...
- Phosphorylation and membrane dissociation of the ARF exchange factor GBF1 in mitosis
[作者:Morohashi, Y; Balklava, Z; Ball, M; Hughes, H; Lowe, M,期刊:Biochemical Journal, 页码:401-412 , 文章类型: Article,,卷期:2010年427-Part 3]
- Secretory protein trafficking is arrested and the Golgi apparatus fragmented when mammalian cells enter mitosis. These changes are thought to facilitate cell-cycle progression and Golgi inheritance, and are brought about...
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