- Properties of a LacY Efflux Mutant
[作者:Guan, L; Kaback, HR,期刊:Biochemistry, 页码:9250-9255 , 文章类型: Article,,卷期:2009年48-39]
- Crystal structures of lactose permease from Escherichia coli (LacY) exhibit two six-helix bundles with 2-fold pseudosymmetry separated by a large hydrophilic cavity. The cavity is open only on the cytoplasmic side and co...
- Peptide Conformer Acidity Analysis of Protein Flexibility Monitored by Hydrogen Exchange
[作者:LeMaster, DM; Anderson, JS; Hernandez, G,期刊:Biochemistry, 页码:9256-9265 , 文章类型: Article,,卷期:2009年48-39]
- The amide hydrogens that are exposed to solvent in the high-resolution X-ray structures of ubiquitin, FK506-binding protein, chymotrypsin inhibitor 2, and rubredoxin span a billion-fold range in hydroxide-catalyzed excha...
- Hydrophobic Photolabeling Studies Identify the Lipid-Protein Interface of the 5-HT3A Receptor
[作者:Sanghvi, M; Hamouda, AK; Davis, MI; Morton, RA; Srivastava, S; Pandhare, A; Duddempudi, PK; Machu, TK; Lovinger, DM; Cohen, JB; Blanton, MP,期刊:Biochemistry, 页码:9278-9286 , 文章类型: Article,,卷期:2009年48-39]
- A HEK-293 cell line that stably expresses mouse 5-HT(3A)Rs containing a C-terminal extension that confers high-affinity binding of alpha-bungarotoxin (alpha BgTx) was established (alpha BgTx-5-HT(3A)Rs) and used to purif...
- Effects of Phospholamban Transmembrane Mutants on the Calcium Affinity, Maximal Activity, and Cooperativity of the Sarcoplasmic Reticulum Calcium Pump
[作者:Trieber, CA; Afara, M; Young, HS,期刊:Biochemistry, 页码:9287-9296 , 文章类型: Article,,卷期:2009年48-39]
- Regulation of the SERCA calcium pump by phospholamban (PLB) is largely due to interactions between their respective transmembrane domains. In spite of numerous mutagenesis and kinetic studies, we still do not have a clea...
- The Chaperone-like Protein alpha-Crystallin Dissociates Insulin Dimers and Hexamers
[作者:Rasmussen, T; Kasimova, MR; Jiskoot, W; van de Weert, M,期刊:Biochemistry, 页码:9297-9304 , 文章类型: Article,,卷期:2009年48-39]
- The protein alpha-crystallin, a member of the small heat shock protein family, has the ability to prevent aggregation of partially denatured proteins, an effect demonstrated both in vivo and in vitro. In this work, we ha...
- TonB Interacts with BtuF, the Escherichia coli Periplasmic Binding Protein for Cyanocobalamin
[作者:James, KJ; Hancock, MA; Gagnon, JN; Coulton, JW,期刊:Biochemistry, 页码:9212-9220 , 文章类型: Article,,卷期:2009年48-39]
- By its direct contact with outer membrane receptor BtuB, the cytoplasmic membrane transducer TonB delivers energy that mediates cyanocobalamin uptake in Escherichia coli. This activity has been generally proposed to be t...
- The Escherichia coli ClpA Molecular Chaperone Self-Assembles into Tetramers
[作者:Veronese, PK; Stafford, RP; Lucius, AL,期刊:Biochemistry, 页码:9221-9233 , 文章类型: Article,,卷期:2009年48-39]
- The Escherichia coli ATP-dependent protease, ClpAP, is composed of the hexameric ATPase/protein-unfoldase, ClpA, and the tetradecameric proteolytic component, ClpP. ClpP proteolytically degrades folded proteins only when...
- The Iron-Sulfur Cluster of Pyruvate Formate-Lyase Activating Enzyme in Whole Cells: Cluster Interconversion and a Valence-Localized [4Fe-4S](2+) State
[作者:Yang, J; Naik, SG; Ortillo, DO; Garcia-Serres, R; Li, M; Broderick, WE; Huynh, BH; Broderick, JB,期刊:Biochemistry, 页码:9234-9241 , 文章类型: Article,,卷期:2009年48-39]
- Pyruvate formate-lyase activating enzyme (PFL-AE) catalyzes the generation of a catalytically essential glycyl radical oil pyruvate formate-lyase (PFL). Purified PFL-AE contains all oxygen-sensitive, labile [4Fe-4S] clus...
- Conformation of the Phosphate D-Alanine Zwitterion in Bacterial Teichoic Acid from Nuclear Magnetic Resonance Spectroscopy
[作者:Garimella, R; Halye, JL; Harrison, W; Klebba, PE; Rice, CV,期刊:Biochemistry, 页码:9242-9249 , 文章类型: Article,,卷期:2009年48-39]
- The conformation Of D-alanine (D-Ala) groups of bacterial teichoic acid is a central, yet untested, paradigm of microbiology. The D-Ala binds via the C-terminus, thereby allowing the amine to exist as a free cationic NH3...
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